Introduces the use instructions of Agilent VariTide RPC Columns, including shipping eluent, column sealing, column conditioning, mobile phase, reverse phase chromatography, flow rate/column pressure, etc.
This document serves as a guide to maximize the performance and lifetime of Agilent VariTide RPC columns, which are optimized for the analysis and purification of synthetic peptides. The columns are physically and chemically stable across the complete pH range and are supplied containing a 7:1 (w/w) acetonitrile/water shipping eluent.
| Shipping Eluent | 7:1 (w/w) acetonitrile/water |
|---|---|
| Maximum Operating Pressure | 3000 psi (207 bar) |
| Optimum Flow Rate | less than 360 cm/h |
| Recommended Tubing ID | 0.010 in (analytical) / 0.020 in (preparative) |
| Filter Requirement | < 0.5 µm |
A 1/16 in stainless steel tubing is recommended, with 0.010 in id for analytical work and 0.020 in id for preparative work.
No, you should never use 100% aqueous eluents as it may reduce column performance when reintroducing organic modifier.
The end plugs must be replaced to prevent the column from drying out, which would disrupt the packed bed.