This document introduces the installation steps of Agilent PL Rapide Aqua Rapid Aqueous SEC Columns, including connecting pipes and connectors, column connections, etc.
This document provides technical data and operational guidelines for the Agilent PL Rapide Aqua series of aqueous SEC columns. It details recommended tubing connections, compatible fittings, and specific procedures for column installation to ensure optimal system performance.
The guide covers critical operating parameters such as eluent selection (including pH limits and organic solvent restrictions), maximum pressure and temperature ratings, and flow rate recommendations based on column dimensions. It also includes instructions for sample preparation, injection volumes, and routine maintenance like reverse flushing frit blockages.
| Typical operating pressure | 225 psi (15 bar) |
|---|---|
| Maximum operating pressure | 2000 psi (140 bar) |
| Maximum operating temperature | 40 °C |
| Efficiency (H2) | > 35,000 plates/m |
The only recommended organic solvent is methanol, up to 50% by volume.
Buffered eluent systems within the pH range of 2-10 may be used.
The column can be reverse flushed at 1.0 mL/min for 1 minute to remove loosely retained material.