The document is the user manual for Agilent PL-SCX HPLC Columns. It describes the use of the product, features and characteristics.
This document provides technical specifications and operational guidelines for Agilent PL-SCX strong cation exchange HPLC columns. It details the optimized pore structure for analyzing biological macromolecules and specifies compatible tubing sizes for microbore, analytical, and preparative applications.
The guide covers column conditioning procedures, including washing out the shipping eluent (0.1 M Na2SO4 and 0.02% sodium azide) and equilibrating with the mobile phase. It also outlines mobile phase compatibility, sample preparation requirements, and protocols for column clean-up and long-term storage.
| Maximum operating pressure | 3000 psi (207 bar) |
|---|---|
| pH range | 1-13 |
| Maximum flow rate | 4.0 mL/min |
| Maximum temperature | 80 °C |
A 1/16 in stainless steel tubing with 0.010 in id is recommended for analytical work.
Yes, both anionic and non-ionic detergents can be used, but the column must be conditioned with the required detergent prior to use.