Agilent Bond Elut Plexa SPE Method Guide

This guide provides a generic method for using Bond Elut Plexa SPE products for solid phase extraction. These methods are effective for separating acids, neutrals and bases from a variety of samples.


Agilent Bond Elut Plexa SPE Method Guide - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
2
File format
PDF
File size
666 KB
Published
04 July, 2012
Updated
30 September, 2023
MD5 checksum
C86D4592234867037DE2D638C7F08BA1

About this manual

This guide provides generic method recommendations for the Bond Elut Plexa family of polymeric SPE products. It covers sample treatment, sorbent conditioning, equilibration, washing, and elution steps for acidic, neutral, and basic analytes with LogP values greater than 0.8.

What's inside

  • Generic method recommendations
  • Sample Treatment
  • Sorbent Condition
  • Equilibration
  • Wash
  • Elution
  • Method development and troubleshooting for plasma samples
  • Non-Polar SPE for neutrals
  • Strong Anion Exchange SPE for Acidic Analytes
  • Strong Cation Exchange SPE for Basic Analytes

Specifications

Sorbent TypePolymeric SPE products / non-polar divinylbenzene-based neutral polymeric sorbent
Particle Size DistributionMonodisperse
Recommended Flow Rate1 mL/min
Standard Device Volume30 mg, 1 mL SPE format

Frequently asked questions

What is the recommended flow rate for elution?

The recommended flow rate is 1 mL/min.

How should basic analytes be adjusted prior to loading on Plexa PCX?

Basic analytes should be at least 2 pH units below the pKa.

What is the standard volume for the methods described?

The volumes are stated for a 30 mg, 1 mL SPE format device.

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