Agilent SureSelect Target Enrichment for 454 Sequencing Product Leaflet

The leading target enrichment platform is now optimized and validated for all major second-generation sequencing platforms. Precisely direct your search for genetic variants with the Agilent SureSelect Target Enrichment System for 454 Sequencing. With protocols that are fast and easy to use, you receive superior on-target performance and read-depth uniformity. Address your specific requirements with a variety of products optimized for 454 Sequencing systems. Enrich for the human exome or kinome with our off-the-shelf kits — or develop your own custom capture product with eArray, which allows you to easily design your own targets from <200 Kb up to 6.8 Mb. Our state-of-the-art, high-fidelity SurePrint long oligo printing facility delivers your order quickly so you can get your research moving faster than with other target enrichment products.


Agilent SureSelect Target Enrichment for 454 Sequencing Product Leaflet - cover page
Brand
Agilent
Category
Other
Document type
Product Leaflet
Language
English
Pages
2
File format
PDF
File size
105 KB
Published
04 July, 2012
Updated
30 September, 2023
MD5 checksum
C4BB00B2DCE2BD47F4F7E5B3103E8642

About this manual

The Agilent SureSelect Target Enrichment System is a leading platform optimized and validated for major second-generation sequencing platforms, specifically the 454 GS FLX Titanium and GS Junior sequencers. The system allows users to precisely direct their search for genetic variants using fast and easy protocols that require 5-10 times less DNA than other protocols and feature a fast 24-hour hybridization process.

What's inside

  • Target Enrichment System
  • SureSelect Custom Capture
  • SureSelect Catalog Kits
  • Human Exome
  • Human Kinome
  • SNP Detection Sensitivity
  • Read Depth Uniformity
  • eArray Custom Design

Specifications

Compatible Sequencers454 GS FLX Titanium and GS Junior
DNA Requirement5-10 times less than other protocols
Hybridization Time24-hour
Custom Capture Size Range<200 Kb up to 6.8 Mb
Average Read Depth (Custom 8.5 Mb)53X
On-Target Reads (Custom 8.5 Mb)60 percent
SNP Detection Sensitivity98 percent of reference SNPs
HapMap Concordance100 percent

Frequently asked questions

Which 454 sequencers are compatible with SureSelect?

The system is fully validated and compatible with 454 GS FLX Titanium and GS Junior sequencers.

How much less DNA is required compared to other protocols?

The SureSelect system requires 5-10 times less DNA than for other protocols.

What is the hybridization time for the SureSelect system?

The system features a fast, 24-hour hybridization process.

Can I design custom targets using eArray?

Yes, eArray allows you to easily design your own targets from <200 Kb up to 6.8 Mb.

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