Agilent AffinityScript One-Step RT-PCR Kit Data Sheet

AffinityScript One-Step RT-PCR Kit is a high-performance single-step RT-PCR kit that can achieve full-length, high-fidelity RT-PCR for targets up to 9.5 kb in length and obtain high yields. This kit is suitable for gene profiling and other high-throughput end-point RT-PCR applications due to its high throughput and rapid running.


Agilent AffinityScript One-Step RT-PCR Kit Data Sheet - cover page
Brand
Agilent
Document type
Product Leaflet
Language
English
Pages
2
File format
PDF
File size
223 KB
Published
04 July, 2012
Updated
30 September, 2023
MD5 checksum
C564E10A30347F5363B87145075DD491

About this manual

The Agilent AffinityScript One-Step RT-PCR Kit is a complete system designed for fast, high-yield, and reliable single-tube reverse transcription PCR applications. It utilizes the multiple temperature reverse transcriptase (AT) which shows optimal activity over a broad temperature range from 42°C to 55°C.

What's inside

  • Broad Temperature Range
  • High Yield Performance
  • Long Template Amplification
  • Multiplex PCR Capability
  • Sensitivity Comparison

Frequently asked questions

What is the maximum length of a target the kit can amplify?

The kit can achieve full-length RT-PCR from targets up to 9.5 kb in length.

What is the optimal temperature range for the reverse transcriptase in this kit?

The reverse transcriptase shows optimal activity over a broad temperature range from 42°C to 55°C.

How much total RNA is routinely required to detect and amplify targets?

The AffinityScript RT routinely detects and amplifies targets using 1 ng of total RNA.

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