The Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip® kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a reporter molecule.
The document details a method for monitoring transfection efficiency in CHO-K1 cells using green fluorescent protein (GFP) as a reporter molecule. It demonstrates that the Agilent 2100 bioanalyzer can accurately quantify the percentage of transfected cells, with results comparable to conventional flow cytometry but requiring significantly fewer cell events.
The system counts approximately 600 to 800 cell events for each sample run on the cell assay chip.
Stained cells must be resuspended at a density of 2 x 10^8 cells/ml in the supplied cell buffer.
The complexes are added to the cells and incubated for 6 hours before the transfection medium is replaced.