This Application Note describes the quantitative analysis of carbohydrates in fruit juices. The analysis technique deployed the Agilent 1200 Series evaporative light scattering detector, which proved to be a viable alternative to refractive index detection because of lower susceptibility to temperature fluctuations and the possibility to perform gradient analyses. Correlation coefficients greater than 0.99 were achieved for the carbohydrates glucose, fructose, sucrose and sorbitol.
This document details a method for analyzing glucose, fructose, sucrose, and sorbitol in commercial fruit juices. The study utilizes the Agilent 1200 Series LC system equipped with an evaporative light scattering detector (ELSD) as an alternative to refractive index detection.
The analysis employs a water/acetonitrile gradient elution on an ALLTECH Prevail Carbohydrate ES column. Results demonstrated high sensitivity and linearity, achieving correlation coefficients greater than 0.99 for the analyzed carbohydrates in orange, grape, pineapple, and apple juices.
The analysis covered sucrose, glucose, fructose, and sorbitol.
The flow rate for the analysis is set to 1 mL/min.