Agilent 1100 Series Application Note: Analysis of Triglycerides in Edible Oils by APCI LC/MS

This application describes a method for analyzing intact triglycerides by LC/MS and the type of information available.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 8
File format: PDF
File size: 291 KB
Published: 02 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: 992D320C264834F924102C563C9E6E05
PDF: Agilent 1100 Series Application Note: Analysis of Triglycerides in Edible Oils by APCI LC/MS PDF

About this manual

This document details an analytical method for characterizing triglycerides in plant oils and animal fats using Liquid Chromatography/Mass Spectrometry (LC/MS). The method utilizes Atmospheric Pressure Chemical Ionization (APCI) to analyze intact triglycerides, avoiding the need for hydrolysis and derivatization required by traditional Gas Chromatography/Mass Spectrometry (GC/MS) methods. The approach allows for the determination of fatty acid composition, degree of unsaturation, and product purity.

What's inside

Introduction
Experimental
Separation and Ionization
LC Conditions
MS Conditions
Qualitative Spectral Information
Results and Discussion
Conclusions

Specifications

Column	Hypersil MOS, 200 mm x 2.1 mm, 5um
Flow rate	0.25 mL/ min
Scan range	300-1100 m/z

Frequently asked questions

What is the primary benefit of this LC/MS method compared to traditional GC/MS?

It offers the ease of LC separation plus the specificity of mass detection without requiring hydrolysis and derivatization.

What is the recommended concentration for dissolving vegetable oils in isopropanol?

The document recommends dissolving 20 uL of oil per 10 mL of isopropanol.

What is the recommended concentration for dissolving animal fat in isopropanol?

The document recommends dissolving 20 mg of fat per 10 mL of isopropanol.

Download PDF (291 KB)