Agilent 1200 Series Aminoglycoside Antibiotics Analysis Application Note

Analysis of Aminoglycoside Antibiotics

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 8
File format: PDF
File size: 307 KB
Published: 02 July, 2012
Updated: 30 September, 2023
Model: 1200 Series
MD5 checksum: 925EC115B7717A4F60C3CCB86091430D
PDF: Agilent 1200 Series Aminoglycoside Antibiotics Analysis Application Note PDF

About this manual

This document details the analysis of aminoglycoside antibiotics, such as gentamicin, kanamycin, and etimicin, using High-Performance Liquid Chromatography (HPLC). The text explains that these compounds are effectively analyzed using an evaporative light scattering detector (ELSD) because they lack a chromophore, making UV detection insufficient. The application focuses on methods compliant with the Chinese Pharmacopoeia (CHP) and includes a specific measurement of etimicin in an injection formulation.

What's inside

Introduction
Experimental
Liquid Chromatographic Separation
HPLC Conditions
System Suitability
Measurement of Etimicin
Calibration Curves
Conclusions

Specifications

System	Agilent 1200 Series SL, binary pump
Detector	Evaporative light scattering detector (ELSD)
Flow rate	1 mL/min
TCC temperature	25°C
ELSD temperature	60°C
ELSD pressure	3.5 bar

Frequently asked questions

Why is an evaporative light scattering detector (ELSD) used instead of UV detection?

ELSD is used because the aminoglycosides do not have a chromophore and UV detection provides insufficient sensitivity.

What is the resolution requirement for kanamycin and kanamycin B?

The resolution between kanamycin and its related compound kanamycin B must be greater than 5.0.

What is the required resolution for netilmicin and etimicin?

The resolution between netilmicin and etimicin must be greater than 1.20.

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