The Agilent PCR Preparation Workstation is a flexible workstation that provides high-throughput PCR preparation. Up to 150 microplates can be processed in one run (with tip changing). The plate processing time is approximately 5 hours for a 50 microplate run, depending on the protocol complexity.
The document outlines a protocol for preparing PCR microplates using the Agilent PCR Preparation Workstation, which combines the Agilent Bravo Platform, BenchCel Workstation, and PlateLoc Sealer. The system is designed to speed up plate preparations and provide walk-away time for genomic applications.
The note details the system layout, including the Bravo Platform with chilled reservoirs and tip-wash station, and the BenchCel Workstation with stackers for storage. It provides a specific 15-step workflow for adding diluent, master-mix, and exon solution to microplates, followed by sealing and storage.
The plate processing time is approximately 5 hours for a 50 microplate run, depending on protocol complexity.
The procedure involves dispensing 25 µL of diluent and 25 µL of master-mix into the microplate.
Each rack in the BenchCel stackers can store up to 65 microplates.