This document describes a one-color gene expression capability developed by Agilent Technologies, as well as the performance and advantages of this system.
Agilent Technologies has developed a one-color gene expression capability based on its dual-mode gene expression system. This platform utilizes the Agilent 60-mer oligonucleotide microarrays and is compatible with both one- and two-color detection systems. The document details the optimization of one-color protocols for specific reagents, hardware, and software components.
| Microarray Type | Agilent 60-mer Whole Human Genome Microarrays |
|---|---|
| Microarray Part Number | G4112A |
| Total Genes/Transcripts | 41,000 |
| Probe Length | 60-mer oligonucleotide |
| Software Version | Feature Extraction 8.5 |
| Scanner Part Number | G2565BA |
| Hybridization Temperature | 65 °C |
| Hybridization Duration | 17 hours |
The cyanine 3 dye was chosen because it is less susceptible to degradation by environmental factors.
A total of 35 microarrays were used, with most analysis conducted using 33 labeling replicates.
Data is extracted using Agilent Feature Extraction software version 8.5 and analyzed using Agilent GeneSpring GX software version 7.3.