This Application Note describes the use of highly sulphonated cyclodextrins for the analysis of chiral analytes. By using small 25 µm id capillaries with an extended pathlength of 125 µm id the Joule heating can be controlled while enhancing sensitivity.
The document details a method for analyzing chiral solutes using capillary electrophoresis (CE) as an alternative to liquid chromatography. It highlights the use of highly sulphonated cyclodextrins, specifically Heptakis (6-sulfato)-R-cyclodextrin supplied by Regis Technologies.
To manage Joule heating caused by high currents associated with these additives, the method utilizes small 25 µm internal diameter capillaries with an extended pathlength. The note demonstrates the generic applicability of this approach to separate various drugs and confirms the stability of thermostatting through reproducibility tests on clenbuterol enantiomers.
The method uses small capillaries with an internal diameter of 25 µm.
The chromatographic conditions specify a voltage of 30 kV.