This document introduces the HPLC method of analyzing antioxidants and UV stabilizers.
This document details a method for analyzing antioxidants and UV stabilizers in polymers using reversed-phase liquid chromatography with diode-array detection. The specific compounds analyzed include Uvinol 3000, Tinuvin P, Irganox 1098, Uvinol 3008, Lavinix BHT, Tinuvin 320, Irganox 1010, Irganox 1076, and Irgafos 168. The method utilizes a BDS column with a mobile phase of water containing tetrabutylammoniumhydrogensulfate and acetonitrile.
| Column | 125 x 3 mm BDS, 3 um |
|---|---|
| Mobile Phase A | Water + 0.001 m Tetrabutylammoniumhydrogensulfate, pH = 3.0 with H,SO, |
| Mobile Phase B | Acetonitrile |
| Gradient | Start with 30 % B, to 98 % Bin 10 min |
| Flow Rate | 0.5 ml/min |
| Injection Vol | 5 |
| Oven Temp | 40 °C |
| UV-Detector | DAD, 280/20 nm |
| Reference | 900/50 nm |
| Limit of Detection | <1ng |
| Precision of retention times | 0.2 % |
| Precision of areas | < 3% |
The analysis uses an Agilent 1100 Series system including a degasser, binary pump, autosampler, thermostatted column compartment, and diode array detector.
The system uses Agilent ChemStation software.
Polymer samples are dissolved in Tetrahydrofurane and filtered after extraction with an ultra-sonic bath for 30 min.