Measuring intraday and interday precision of GPC-SEC analysis data
This document details the precision of molecular weight data (Mw and Mn) obtained via Gel Permeation Chromatography (GPC-SEC) using the Agilent 1100 Series system. It highlights that modern equipment allows for significantly improved precision compared to earlier techniques. The note explains that precision is heavily influenced by hardware stability, specifically flow rate deviations and column temperature stability, as well as software integration algorithms.
| Refractive Index Detector noise | + 2.5 x 10-9 RIU |
|---|---|
| Refractive Index Detector drift | 200 x 10° RIU/h |
The interday precision for retention time was 0.069% over 20 days.
The intraday precision was always below 0.05%.
A 4 °C change in column temperature creates an error of 2.6% in the molecular weight data.