An LC/MS/MS method was developed for the qualitative and quantitative measurement of nitrofuran metabolites in chicken and shrimp using the Agilent LC/MSD XCT Ion Trap. The limit of quantitation for all four nitrofurans investigated easily met the specified European Union Minimum Required Performance Level of 1 µg/kg and ranged from 0.125 µg/kg to 0.5 µg/kg. Nitrofuran antibiotics are widely used for the treatment of infectious diseases in cattle, pigs, shrimp, and poultry. Due to their rapid metabolism nitrofuran parent substances are not suitable for monitoring and typically their metabolites are analyzed. A liquid chromatography /mass spectrometry/mass spectrometry (LC/MS/MS) method was developed for the sensitive, qualitative, and quantitative analysis of four derivatized nitrofuran metabolites found in shrimp and poultry.
The document details a developed Liquid Chromatography/Mass Spectrometry/Mass Spectrometry (LC/MS/MS) method designed for the qualitative and quantitative measurement of four derivatized nitrofuran metabolites in chicken and shrimp. The study utilizes an Agilent 1100 Series LC system coupled to an Agilent LC/MSD Trap XCT Ion Trap mass spectrometer.
The limit of quantitation for all four nitrofurans investigated ranged from 0.125 ug/kg to 0.5 ug/kg.
One (1) g of homogenized tissue from shrimp or poultry was mixed with reagents for the procedure.
The mixture must be incubated overnight at 37 °C to release tissue-bound residues.