EtG is a minor metabolite of ethanol, can be detected in blood for up to 36 hours and in urine for up to 5 days, can be used as a biomarker for alcohol consumption. This application note describes a fast and sensitive EtG analysis method using the Varian Pursuit C18 HPLC column and 320-MS LC/MS/MS system, which demonstrates good linearity, high sensitivity, and stable ion ratios with a detection limit of 10 ppb.
This document details a method for detecting 8-ethyl glucuronide (EtG), a biomarker for alcohol consumption, in urine samples. The method utilizes the Varian 320-MS LC/MS/MS system combined with Varian Pursuit C18 HPLC columns to achieve high sensitivity and fast separation times.
The analysis demonstrates detection limits of 10 ppb with a reporting limit of 52 ppb. The method involves direct injection of spiked urine samples and utilizes specific ion ratios for quantitation, achieving separation within 2 minutes. The document provides details on instrumentation, materials, HPLC conditions, and results including calibration curves and fragment ion data.
| Detection Limit | 10 ppb |
|---|---|
| Reporting Limit (LOD) | 52 ppb |
| Reporting Limit (LOQ) | 152 ppb |
| Column Type | Pursuit C18 5 um, 50 x 4.6 mm ID |
| Flow Rate | 0.7 mL/min |
| Calibration Linearity | r² greater than 0.999 |
The detection limit of 10 ppb can be easily reached.
The method uses a Varian 320-MS LC/MS/MS system with Varian 212-LC Binary Gradient LC/MS Pumps and a Varian Combi PAL AutoSampler.