Agilent EnviroPrep Columns Loading Study Application Note

About this manual

This document presents an application note detailing a loading study performed on Agilent EnviroPrep preparative GPC columns. The study utilized a mixture of four test probes (Corn oil, Bis(2-ethylhexyl) phthalate, Methoxychlor, and Perylene) to assess the separation of small molecules from a high molecular weight matrix under varying sample concentrations.

What's inside

• Introduction
• Materials and Methods
• Conditions
• Results and Discussion
• Conclusion

Frequently asked questions

What is the maximum on-column loading for the EnviroPrep columns tested in this study?

The chromatography gave good peak shapes up to 10.0 mg on-column loading.

What sample concentration range was used for the individual samples in the loading study?

The samples were injected individually to produce on-column loadings of 2.5, 5.0 and 10.0 mg.

Which specific sample showed a consistent change in chromatography as the concentration increased?

The methoxychlor sample displayed a negative dip after the main peak as the concentration was increased.