This document describes a method for the determination of platinum and palladium in chelated systems using GFAAS.
This document details the determination of platinum and palladium in chelated systems, specifically cisplatin and related compounds, using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). The study focuses on measuring the stability constants for Pt-DNA and Pd-DNA complexes, utilizing the labile nature of palladium as a model for platinum.
The procedure employs an Agilent SpectrAA 400 Atomic Absorption Spectrometer equipped with a GTA96 Graphite Tube Atomizer. Specific furnace parameters, including ashing and atomization temperatures, are optimized for both elements. The note also compares the use of premixed standards versus the autosampler's automix facility, demonstrating comparable calibration results and reduced operator error.
| Instrument Model | Agilent SpectrAA 400 Atomic Absorption Spectrometer |
|---|---|
| Atomizer Model | GTA96 Graphite Tube Atomizer |
| Platinum Resonance Line | 265.0 nm |
| Platinum Slit Width | 0.2 nm |
| Palladium Resonance Line | 244.8 nm |
| Palladium Slit Width | 0.3 nm |
| Lamp Current | 5 mA |
| Platinum Atomization Temperature | 2700 °C |
| Palladium Atomization Temperature | 2600 °C |
An Agilent SpectrAA 400 Atomic Absorption Spectrometer and GTA96 Graphite Tube Atomizer were employed.
The optimal atomization temperature chosen for platinum is 2700 °C.
Yes, the automix facility provides a calibration graph that is comparable with that from premixed standards.