This application note describes the separation of 4 mycotoxins using HPLC with the Agilent Pursuit XRs Ultra 2.8 Diphenyl column. The elution was isocratic, the flow rate was 0.4 mL/min, the temperature was 40 °C, and the detection wavelength was 236 nm.
This document details the separation of mycotoxins, specifically beta-Zearalenol, alpha-Zearalenol, Zearalanone [IS], and Zearalenone, using the Agilent Pursuit XRs Ultra 2.8 Diphenyl column. The method utilizes isocratic elution with a fast run time.
| Column | Agilent Pursuit XRs Ultra 2.8 Diphenyl, 2.0 x 100 mm, 2.8 ym |
|---|---|
| Sample size | 1 uL |
| Sample concentration | 1 g/mL |
| Sample solvent | Acetonitrile |
| Mobile phase A | 0.005 M ammonium acetate, 0.1% methanol, 0.02% formic acid, pH 3.6 |
| Mobile phase B | methanol |
| Gradient | Isocratic A:B 40:60 |
| Flow rate | 0.4 mL/min |
| Temperature | 40°C |
| Pressure | 180 bar |
| Detector | UV, 236 nm |
The method separates beta-Zearalenol, alpha-Zearalenol, Zearalanone [IS], and Zearalenone.
The flow rate used is 0.4 mL/min.
The detector is set to UV at 236 nm.