Agilent Fast LC/MS/MS Analysis of Group 4 Pharmaceuticals from EPA-1694 with RRHD HILIC Plus Application Note

This application note describes a method for accelerating the analysis of Group 4 pharmaceuticals in EPA-1694 using an Agilent 1290 Infinity UHPLC and an Agilent ZORBAX RRHD HILIC Plus column. All compounds provide excellent peak shapes, while the flow rate and sample throughput are increased by four times the original rate.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 77 KB
Published: 11 June, 2012
Updated: 30 September, 2023
MD5 checksum: EFB4BFB2683E53E660183771B0EA948F
PDF: Agilent Fast LC/MS/MS Analysis of Group 4 Pharmaceuticals from EPA-1694 with RRHD HILIC Plus Application Note PDF

About this manual

This document details a method for analyzing Group 4 compounds (cimetidine, albuterol, ranitidine, and metformin) found in EPA-1694. The analysis utilizes an Agilent 1290 Infinity UHPLC system coupled with an Agilent ZORBAX RRHD HILIC Plus column to achieve high throughput.

What's inside

Abstract
Introduction
Experimental
Results and Discussion
Conclusions

Frequently asked questions

What is the maximum pressure the Agilent ZORBAX RRHD HILIC Plus column can withstand?

The column is stable to pressures up to 1200 bar.

How much does the analysis time decrease when using the RRHD HILIC Plus column compared to the typical run?

The run time is reduced by 75%.

What is the flow rate used in the experiment described in this application note?

The flow rate used is 1 mL/min.

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