Agilent Identification of different meat species by the Agilent Fish ID solution on the Agilent 2100 Bioanalyzer Manual

About this manual

This document presents an application note investigating the ability of the Agilent Fish ID solution to detect non-fish species, specifically mammalian or avian DNA, in meat samples. The study utilized beef, pork, wild boar, lamb, chicken, turkey, and duck samples to validate the detection capabilities of the Fish ID Ensemble and PCR-RFLP Fish ID kit.

What's inside

• Introduction
• Materials and Methods
• Meat samples
• Isolation of DNA
• Amplification of Cyth target sequence
• Restriction digestion
• Analysis of restriction patterns
• Results
• Summary

Specifications

Frequently asked questions

Which meat species were tested in this study?

The study tested beef, pork, wild boar, lamb, chicken, turkey, and duck samples.

What types of meat samples were tested to validate the solution?

The study tested samples of beef, pork, wild boar, lamb, chicken, turkey, and duck.

How many independent DNA samples were used for each meat tissue?

Two independent DNA samples were used for each of the meat tissues.

What is the maximum number of samples that can be analyzed per chip?

Up to four samples can be analyzed per chip.