This application note describes a method for analyzing fullerenes using Agilent PLgel columns and gel permeation chromatography.
Fullerenes are typically prepared by evaporating graphite rods in arc reactors, resulting in soot containing various fullerene compounds. The soluble fractions can be extracted using a suitable solvent and further purified via gel permeation chromatography (GPC). Low pore size Agilent PLgel 50A GPC columns are recommended for these analytical applications to screen fullerene compounds.
| Eluent | Toluene |
|---|---|
| Flow Rate | 1.0 mL/min |
| Loading Concentration | 2 mg/mL |
| Detector Type | RI |
| System Model | Agilent PL-GPC 50 |
Toluene is recommended because it provides reasonable solubility, good chromatographic selectivity, and is amenable to solvent removal.
Toluene is the recommended solvent because it provides reasonable solubility, good chromatographic selectivity, and allows for easy removal by distillation.
The recommended flow rate for the described analytical separation is 1.0 mL/min.
The procedure requires a loading of 2 mg/mL with an injection volume of 200 pL.