This article describes the analysis of oligosaccharides using gel permeation chromatography on Agilent PLgel. NMP is used as the solvent, allowing for the analysis of more polar oligosaccharides such as starch. The viscosity of NMP is extremely high, and operating at elevated temperature is recommended to reduce pressure and enhance resolution.
Oligosaccharides are naturally occurring saccharides that can be analyzed using gel permeation chromatography. This document demonstrates a method for analyzing these compounds in polar organic solvents like N-methylpyrrolidone (NMP). The high viscosity of NMP requires operation at elevated temperatures to reduce pressure and enhance resolution.
The recommended conditions include the use of Agilent PLgel 5 µm 500A columns with a length of 7.5 x 300 mm. The analysis utilizes an RI detector, a flow rate of 1.0 mL/min, and a temperature of 80 °C.
| Column Part Number | PL1110-6525 |
|---|---|
| Flow Rate | 1.0 mL/min |
| Temperature | 80 °C |
The document demonstrates the use of N-methylpyrrolidone (NMP) as a polar organic solvent.
Elevated temperature is recommended to reduce pressure and enhance resolution due to the high viscosity of NMP.