Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
This document details how the Agilent 2100 bioanalyzer can be used to analyze apoptotic cell samples, comparing its performance with conventional flow cytometry. It demonstrates that the microfluidic system provides data quality in good agreement with traditional methods while requiring fewer cells.
The note provides detailed protocols and reagent recommendations for two specific assays: an annexin V assay to detect phosphatidyl serine exposure and a caspase-3 assay to measure active proteases. It highlights the system's advantages, including high reproducibility, low cell consumption, and ease of use.
The system offers high reproducibility, low cell consumption, and ease-of-use.
The histogram quality of fluorescent cells counted with the microfluidic system is in good agreement with data obtained with a flow cytometer.