This document describes the method of high performance DNA oligonucleotide purification using Agilent TOP-DNA. Synthetic oligonucleotides have become readily available due to solid phase synthesis, but there are some limitations such as the need for high coupling yields and difficulties in purification. Agilent has developed a purification method specifically designed for DNA oligomers up to 150 bases.
This document details a high-performance purification method for synthetic DNA oligonucleotides using Agilent's TOP-DNA system. The technique utilizes trityl-on purification with solid phase extraction (SPE) tubes to separate full-length products from failure sequences, specifically designed for oligomers up to 150 bases in length.
| Maximum oligomer length | 150 bases |
|---|---|
| Tube capacity | 96 oligonucleotides |
| Purification time | 10 to 15 minutes |
| Tube part number | 7572915C |
| Manifold part number | 12234100 |
| Baseplate part number | 75700001 |
The method is applicable to DNA oligomers up to 150 bases in length.
The system can purify as few as one or as many as 96 DNA oligonucleotides.
The purification process takes approximately 10 to 15 minutes if carried out without breaks.