This document introduces the Agilent TOP-RNA high-performance RNA oligonucleotide purification technology
This document details a method for purifying synthetic RNA oligonucleotides using Agilent's TOP-RNA solid phase extraction tubes. The protocol addresses the limitations of traditional DNA purification methods when applied to RNA, specifically focusing on the removal of failure sequences and the de-silylation of trityl-on oligomers. The method is designed to process up to 96 samples in less than 20 minutes using a vacuum procedure.
| Tube capacity | 100 mg |
|---|---|
| Tube format | 96/pk |
| Processing time | less than 20 minutes |
| Required baseplate | Agilent VersaPlate |
| Yield | > 85% |
| Purity | > 90% |
The method can purify as few as one, or as many as 96 RNA oligonucleotides.
The vacuum should be set to 7 in Hg.
The purification takes approximately 10 to 15 minutes if carried out without breaks.