ASAPHMQC is a fast multidimensional NMR experiment that provides both one-dimensional and two-dimensional spectra to help analyze the composition and structure of chemical substances.
This application note introduces the Acceleration by Sharing Adjacent Polarization Heteronuclear Multiple Quantum Coherence (ASAPHMOC) experiment. It highlights that this technique provides information regarding one-bond connectivity and heteronuclear chemical shifts for protonated carbons, similar to HSQC, but requires significantly less time. The document demonstrates the efficiency of ASAPHMOC by comparing it to gradient heteronuclear single quantum coherence (gHSOCAD) data acquired on a sample of clindamycin HCl.
| Default Relaxation Delay | 60 milliseconds |
|---|---|
| Default Mixing Time | 25 milliseconds |
| Default Increments | 96 increments |
| Default Acquisition Time | 64 milliseconds |
| Default Coupling Constant | 146 Hz |
| Default Spectral Width | 170 ppm |
The total experiment time is 56 seconds.
ASAPHMOC provides signals for all protonated carbons in 56 seconds, whereas gHSOCAD required 8.25 minutes.
VnmrJ 3 software provides the easy-to-use, interactive tools for setting up this experiment.