This document describes the application of Agilent RapidFire/MS Systems in high-throughput drug discovery, as well as its advantages in screening challenging targets. The authors use the acetyl-coenzyme A carboxylase (ACC) assay to illustrate the power of this system.
This document describes an application note detailing the analysis of Acetyl-Coenzyme A Carboxylase (ACC) using Agilent RapidFire/MS Systems. The text explains that the RapidFire High-throughput Mass Spectrometry System provides drug discovery researchers with mass spectrometry-based high-throughput screening solutions for targets that are challenging to screen using conventional approaches. It highlights the system's ability to provide label-free native analyte detection, eliminating the need for cumbersome and costly labeling methods.
The system was used to confirm inhibition by myristoyl-CoA, palmitoyl-CoA, and stearoyl-CoA.