This paper introduces the influence of silica particle size and pore size on insulin separation. The results show that a larger pore size and a smaller particle size lead to better separation of insulin. Agilent Poroshell 120 column with pore size (120Å) and superficially porous particle size (2.7µm) is the best choice for insulin analysis.
The document investigates how different column parameters affect the analysis of porcine insulin, a small protein molecule with a molecular weight of about 5800 Da. It compares traditional totally porous silica columns with superficially porous (Poroshell) columns to determine optimal configurations for high efficiency and resolution while meeting regulatory requirements.
A pore size larger than 100A is sufficient; a macro pore size of 300A is not necessary.
The Agilent Poroshell 120 columns with a pore size of 120A and superficially porous particle size of 2.7 µm.
Using smaller particle sizes, such as 1.8 µm instead of 5 µm, results in higher efficiency.