Scalability of Agilent Columns Across HPLC and UHPLC Instruments Application Note

About this manual

The document discusses the transfer of liquid chromatography (LC) methods between various Agilent and non-Agilent systems. It highlights that while instrumentation improves, transferring LC methods requires careful consideration of column dimensions, particle sizes, and system parameters like delay volume to maintain selectivity.

The note presents experimental results using several Agilent ZORBAX columns (Eclipse Plus C18, RRHD Eclipse Plus C18, and Poroshell 120 EC-C18) on instruments ranging from the 1100/1200 Series to the 1290 Infinity LC. It details how gradient scaling equations can be used to optimize methods when changing column length or particle size, and explains the impact of system delay volume on peak elution.

What's inside

• Abstract
• Introduction
• LC Method Parameters
• Gradient Scaling
• Instrument <> Column Compatibility Considerations
• Critical Parameters of Agilent LC Systems
• Results and Discussion
• Effect of System Delay Volume

Specifications

Frequently asked questions

What is the formula for scaling gradient time?

The new gradient time (t2) can be calculated using the equation: t2 = (t1 * d2^2 * L0 * F1) / (d1^2 * L1 * F2).

What is the maximum pressure limit for the Agilent 1290 Infinity LC system?

The maximum pressure limit is 1200 bar.