This Application Note describes purification of poly- and monoclonal antibodies from different media backgrounds constructed to mimic those encountered during antibody screening, production, and scale-up in commercial settings.
The document details a study demonstrating the ability of the Agilent AssayMAP Bravo platform to automate the purification of antibodies. The system utilizes specially formulated microchromatography cartridges containing immobilized protein A to process samples from various media backgrounds, including phosphate buffered saline and cell culture supernatants supplemented with fetal bovine serum.
This document describes the purification of poly- and monoclonal antibodies from different media backgrounds using the Agilent AssayMAP Bravo platform.
The protocol elutes antibodies using 12 mM HCl.
The pipetting head is capable of moving fluids at rates as high as 500 y!L/second.