This document introduces the use of Agilent RapidFire/MS Systems for high-throughput screening. By detecting native analytes without the need for labeling, this technology overcomes the challenges of small substrates and difficult-to-detect modified products in traditional methods, thus converting low-throughput intractable assays into high-throughput assays similar to plate-based optical methods. Using stearoyl-coenzyme A desaturase (SCD) as an example, the power of Agilent RapidFire/MS Systems for screening intractable targets is demonstrated.
This document describes an application of the Agilent RapidFire High-throughput Mass Spectrometry System for analyzing the Stearoyl-Coenzyme A Desaturase (SCD) assay. The RapidFire technology enables label-free native analyte detection, which is particularly useful for targets where substrates and products are too small to label or undergo difficult modifications. This approach eliminates the need for cumbersome and costly labeling methods, allowing traditionally low-throughput assays to be processed at speeds approaching plate-based optical methods.
| Sample processing speed | 6-10 seconds |
|---|---|
| Throughput increase | more than 10-fold |
| Average Z' score | 0.597 |
| Median Z' score | 0.60 |
| Confirmed active inhibitors | 346 |
It provides label-free native analyte detection, eliminating the need for cumbersome and costly labeling methods.
It increases throughput by more than 10-fold by processing samples at speeds of 6-10 seconds.
The average Z' score was 0.597 based on a set of 518 plates.