Agilent Technologies Fast efficient HLA typing analysis using D1K ScreenTape

About this manual

The document details how the Agilent 2200 TapeStation system enables simple and convenient analysis of PCR sequence specific primer (SSP) amplicons for HLA genotyping. It highlights that allele-specific PCR combined with automated electrophoresis allows for rapid detection, interpretation, and storage of tissue typing results.

What's inside

• Abstract
• Introduction
• Experimental Material
• PCR assay
• D1K ScreenTape analysis procedure
• Results and Discussion
• Conclusion

Frequently asked questions

How long does the amplicon separation, imaging, and sizing process take?

The analysis separates, images, and sizes the amplicons within 10 minutes.

What fragment sizes are used as lower and upper markers in the D1K sample buffer?

The buffer contains markers at 25 bp and 1500 bp for accurate sizing.

What is the size of the control amplicon included in each PCR-SSP reaction?

Each reaction contains a control amplicon that is approximately 607 bp.