This application note describes an analytical method for determination of carbendazim and 3 other fungicide residues in orange juice with easy sample preparation and LC/MS/MS detection. Developed for the 3 most important systemic fungicides, carbendazim, thiabendazole, and imazalil with its metabolite, this method can be easily expanded to include a broader range of pesticides. A simple and rapid extraction procedure provides clean extracts that without any preconcentration allow for detection of analytes at concentrations below 2 ppb. Quantification was performed using triphenyl phosphate as an internal standard. Excellent accuracy (% recovery) and precision (% CV) data were obtained for three concentration levels: 10, 50, and 250 ppb of each analyte.
The document presents a simple extraction procedure using Agilent Bond Elut Plexa cartridges to determine fungicide residues in fruit juice. The method allows for the detection of analytes at concentrations below 2 ppb without preconcentration, utilizing triphenyl phosphate as an internal standard.
Quantification is performed using an Agilent 6460 Triple Quadrupole LC/MS system with an ESI source. The analysis utilizes a superficially porous Agilent Poroshell 120 EC-C18 column to achieve separation within approximately 5 minutes, ensuring excellent sensitivity and low matrix interference.
The method determines carbendazim, thiabendazole, imazalil, and the imazalil metabolite.
The actual pressure in the LC system does not exceed 400 bar.
Samples should be centrifuged for 15-20 minutes at 6000 rpm.