Agilent CE System Quick Start Guide to Maintenance and Troubleshooting

About this manual

This document serves as a quick start guide for the operation and care of Agilent Capillary Electrophoresis (CE) systems. It outlines practical considerations regarding capillaries, samples, buffers, and data analysis to ensure reproducibility in mobility, migration time, and peak area.

The manual provides detailed instructions on preventive maintenance for key components such as the injection system, replenishment system, and vial caps. Additionally, it offers troubleshooting advice and a structured approach for CE method development and optimization.

What's inside

• Practical Considerations
• Factors Affecting Reproducibility of Mobility and Migration Time
• Factors Affecting Peak Area Reproducibility
• Preventive Maintenance: Injection System
• Preventive Maintenance: Replenishment System
• CE Method Development
• CE Method Optimization

Specifications

Frequently asked questions

How often should electrodes and prepunchers be cleaned?

They should be cleaned frequently, for example weekly.

What type of caps are recommended for resealing vials?

Polyurethane caps with part number 5181-1512 should be used.

What is the recommended order for adjusting variables during method optimization?

The order is: 1. Optimize pH, 2. Increase buffer concentration, 3. Change buffer ion, 4. Add buffer modifier or use coated capillary.