Agilent AdEasy Virus Purification Kit 500 Instruction Manual

The AdEasy Virus Purification Kit 500 is a kit used for virus purification. It is for research use only and not for use in diagnostic procedures. The kit offers efficient virus purification and comes with a limited product warranty.


Agilent AdEasy Virus Purification Kit 500 Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
14
File format
PDF
File size
186 KB
Published
06 June, 2012
Updated
28 September, 2023
MD5 checksum
38FF37D61DA6619899070F18CDD5D922

About this manual

The Agilent AdEasy Virus Purification Kit 500 is designed for the rapid purification and concentration of adenovirus (specifically Ad5 strains) from infected cell cultures. The kit includes Sartobind syringe filters with ion exchange membranes, centrifuge concentrators, and necessary buffers to facilitate a protocol that yields purified viral particles in approximately 2-3 hours, significantly faster than traditional CsCl gradient methods.

What's inside

  • Materials Provided
  • Storage Conditions
  • Additional Materials Required
  • Overview of the AdEasy Purification Kit Protocol
  • Purification of Adenovirus Protocol
  • Virus Culture
  • Sample Preparation
  • Sartopore Filter Clarification
  • Sartobind Filter Unit Purification
  • Troubleshooting

Specifications

Viral Particle Yield1-3 x 10^9 particles
Process Time2-3 hours

Frequently asked questions

What is the maximum loading rate for the Sartobind unit?

For maximum binding of virus, load at no more than 10 ml/minute.

How long does the purification process take compared to traditional methods?

The AdEasy method takes 2-3 hours, whereas CsCl gradient centrifugation typically takes 12-48 hours.

What is the recommended storage temperature for purified virus?

Aliquot and store virus at -80°C. Once thawed, keep at 4°C and do not re-freeze.

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