This file is a guide to select Ni Sepharose and IMAC Sepharose products for the purification of histidine-tagged proteins.
This guide presents GE Healthcare’s Ni Sepharose and IMAC Sepharose product families, describing their high‑capacity, low‑cost chromatography media for purifying histidine‑tagged proteins directly from unclarified cell lysates. It outlines the different pre‑packed columns, pre‑charged resin formats, and compatible buffer kits.
The document includes decision tables based on protein amount, sample clarity, and purification goals (high resolution, large scale, high flow). It also lists available column sizes (1 ml, 5 ml, 20 ml, 96‑well plates) and provides contact information for ordering and technical support. This manual is written in Chinese.
| Maximum protein load (high capacity) | >100 mg |
|---|---|
| Minimum protein load (low capacity) | <100 mg |
| Supported metal ions | Ni²⁺, Co²⁺, Zn²⁺, Cu²⁺ |
For protein amounts greater than 100 mg, the guide recommends using HiTrap IMAC HP or HiPrep IMAC FF 16/10 columns.