Industrial

This file describes an LC/MSD autotuning method that can optimize analysis parameters in both positive and negative ionization modes and create a tuning file that contains optimized parameters.

The method used SDB-RPS disk and FMOC for the detection of anatoxin-a. The recovery rate was 97.6%-102.1% at 10 ng/L, and the detection limit was 0.8 ng/L.

This Application Note describes the experimental details and results of a plasma protein binding (PPB) assay. The approach and methodology of rapid resolution liquid chromatography and triple quadrupole mass spectrometry used in this study are described in a separate Application Note1. Experimental details and results of other typical assays per- formed in early ADME profiling of new hits are presented in separate publications2,3.

This Application Note demonstrates how the Agilent LC/MS/MS solution can increase productivity through improved performance of the analytical equipment and superior data analysis software that reports relevant parameters directly from the analytical data. Features of the Agilent solution are: Fast LC methods and short cycle times through alternating column regeneration State-of-the-art MS performance Easy-to-learn and easy-to-use acquisition and data analysis software Calculation of PK parameters directly by the data analysis software Software compliant with the regulations for clinical trials.

This Application Note describes the experimental details and results of a Caco-2 permeability (Caco-2) assay. The approach and methodology of rapid resolution liquid chromatography and triple quadrupole mass spectrometry used in this study are described in a separate Application Note1. Experimental details and results of other typical assays performed in early ADME profiling of new hits are presented in separate publications,2,3. Improving productivity in the determination of parameters for early in vitro ADME Part 3 – Experimental details and results of Caco-2 permeability assay.

This Application Note describes how the BioTrove RapidFire high-throughout mass spectrometry (RF-MS) system interfaced to an Agilent 6410 triple quadrupole mass spectrometer was used for the characterization of the enzymatic reaction of CYP450 3A4/5 enzyme in a preparation of human liver microsomes and for the determination of IC50 curves of poten- tially inhibiting drug compounds.

This study examines the effects of precursor and fragment ion mass accuracy on protein identification using the Accurate Mass Q-TOF. Results demonstrate that fragment ion mass accuracy is as important (if not more important) than precursor ion mass accuracy in reducing false positive identification rates in MS/MS database searches, and plays a critical role in improving the throughput of proteomics research projects.

This paper introduces a new high-capacity affinity column for the removal of 14 high-abundant proteins from human biological samples. This column can effectively remove 14 high-abundant proteins, thereby improving the detection sensitivity of low-abundance proteins.

This application note introduces the Agilent Multiple Affinity Removal System for depletion of six high-abundance proteins from human serum

This document introduces high capacity affinity depletion column technology, which can highly specifically deplete the top six high-abundance proteins in human serum, which is advantageous for the detection and identification of low-abundance proteins.

This Application Note illustrates the potential of combining protein depletion using the Agilent Multiple Affinity Removal System and protein or peptide fractionation using the Agilent 3100 OFFGEL Fractionator for the LC-MS based analysis of plasma proteins.

This is an application note that describes a protein profiling approach that combines accurate mass ESI-TOF data with differential analysis to highlight and identify putative biomarkers.

Here, we present a novel and highly robust method for the separation and identification of HeLa cell membrane proteins by an LC-only based separation strategy. Employing optimized reversed phase (RP) conditions and using a uniquely designed reversed-phase column material specifically engineered to enable high efficient separation of membrane proteins, the results demonstrate that this approach allows high identifications of membrane proteins.

This document illustrates the effectiveness of the Agilent 3100 OFFGEL Fractionator for the prefractionation of complex protein or peptide mixtures. The tryptic digest of E. coli proteins was fractionated into 24 fractions, and peptides were identified using reversed phase liquid chromatography with mass spectrometric detection. The distribution of peptides and their isoelectric points in each fraction was analyzed. The results show efficient peptide fractionation and improved protein identification using the Agilent 3100 OFFGEL Fractionator.

This is an application note that describes a high recovery HPLC method for the identification of human brain lipid raft membrane proteins based on a macroporous reversed-phase column.