Industrial

The Varian Evaporative Light Scattering Detector is a powerful tool for detecting any sample that is less volatile than the mobile phase in HPLC applications, irrespective of the optical properties of the compounds of interest. The ELSD offers distinct advantages over more conventional UV or DAD detection, particularly for gradient separation. In the past, one disadvantage for some ELSD designs has been that high operating temperatures required to fully evaporate the eluent can cause loss of semi-volatile, small molecules, e.g. drug candidates. The Varian Evaporative Light Scattering Detector features design improvements to overcome these drawbacks. For the detection of semi-volatile components in a sample, the Varian ELSD can be operated with the heaters off (ambient temperature operation) or with the nebulizer and evaporator controlled at low temperatures. Figure 1 shows a separation of a test mixture of four compounds by reversed phase HPLC, which is typically used to assess HPLC system performance for pharmaceutical analysis.

Varian Evaporative Light Scattering Detector is a technology that enhances LC-MS and can detect compounds that have little or no UV chromophore.

This document introduces the advantages of Varian OneNMR™ probe, including excellent lineshape performance, superior B1 field homogeneity and outstanding salt tolerance. The probe uses a solvent suppression technique called “Presaturation Utilizing Relaxation Gradients”, or PURGE, which has very efficient and robust solvent suppression effects, excellent frequency selectivity, and no baseline perturbations outside of the suppression window.

This document demonstrates the reproducibility and reliability of the Varian 320-MS triple quadrupole mass spectrometer in analyzing complex samples. By using the 212-LC binary gradient LC/MS chromatography pumps, the instrument can achieve excellent reproducibility of both area and retention time over multiple injections. The method was tested on a heavy matrix containing multiple components and transitions, showing excellent reliability and reproducibility of the system. The document also describes the specific steps and experimental conditions for analyzing samples extracted from tomato, orange, spinach, sweet corn, and raisin matrices using the QUECHERS method. The experimental results show that the % RSD of Buprofezin (306 ➔ 201) retention time is 0.02 and 0.09 over 60 and 100 subsequent injections, respectively, demonstrating the reliability of the system.

This document introduces Varian 320-MS, a triple quadrupole mass spectrometer with a unique 180° off-axis collision cell and triple quadrupole configuration. This instrument can eliminate chemical background noise and cross-talk when similar product ions in complex matrices are monitored in the same time segment.

Varian's 320-MS triple quadrupole system meets the quality guidelines for qualifier assessment and ion ratio stability set by the European Union for the detection and analysis of toxic compounds.

This is a research article that studied the performance of two polymeric strong cation exchange SPE products, the Bond Elut PCX and SampliQ SCX, to determine which product performed better in the retention of acidic, neutral, and basic components in human plasma.

SmartRinse is a feature in the ICP Expert II software that speeds up sample wash-out, reduces carry-over, and increases productivity.

The document introduces an optimized ICP-OES torch for analyzing samples with high total dissolved solids content. The use of this torch provides prolonged instrument stability and lower detection limits. Additionally, it eliminates the need for periodic recalibration and sample dilution, increasing productivity.

The Varian Evaporative Light Scattering Detector provides highly consistent results in peak area response, regardless of the molecular weight of the sample. Accurate and reproducible peak areas are essential when quantifying a sample using a calibration of response versus concentration.

Varian 380-LC series is a range of advanced evaporative light scattering detectors from Polymer Laboratories, now a part of Varian, Inc. The Varian 385-LC delivers sub-ambient evaporation down to 10 °C, providing maximum sensitivity for compounds with significant volatility below ambient temperature. The Varian 380-LC is the instrument of choice for non-volatile compounds, where temperatures of 100 °C or above are required. Both instruments benefit from fast data output rates and extremely low dispersion for fast LC, and deliver a universal response down to the low nanogram range for truly representative analysis. Repeatability is less than 2% for improved consistency of results. Real-time gas control eliminates solvent effects to provide a constant response across a gradient. Control and digital data collection come as standard for multi-vendor platforms and so there is no need for an analog to digital converter. On-the-fly adjustment of light source intensity also saves time during a run, too - all in the smallest footprint around. Being complementary to LC/MS, and offering unrivalled flexibility and sensitivity, the Varian 380-LC series are the ELSDs of choice for the most demanding applications.

The Varian 385-LC is an advanced evaporative light scattering detector from Polymer Laboratories, now a part of Varian, Inc. The Varian 385-LC delivers sub-ambient evaporation down to 10 °C, providing maximum sensitivity for compounds with significant volatility below ambient temperature. The instrument benefits from fast data output rates and extremely low dispersion for fast LC, and delivers a universal response down to the low-nanogram range for truly representative analysis. Reproducibility is less than 2% for improved consistency of results. The Varian 385-LC offers real time gas management that eliminates solvent effects to give a constant response across a gradient. Control and digital data collection come as standard for multi-vendor platforms and so there is no need for an analog to digital converter. On-the-fly adjustment of light source intensity saves time during a run, too - all this in the smallest footprint around. Being complementary to LC/MS, and offering unrivalled flexibility and sensitivity, the Varian 385-LC is the ELSD of choice for the most demanding applications. The success of ELS detection relies on evaporating the eluent without destroying the analyte particle. Where the analyte is non-volatile, evaporator temperatures of 50-100 °C can be used without compounds degrading, thus giving maximum sensitivity. However, when compounds have high vapor pressures and the eluent has a high boiling point (eg water), since the evaporation rate of the eluent is much lower than that of the analyte, the light scattering signal will be reduced. The Varian 385-LC uses a unique condenser design to provide high sensitivity in this case.