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This document provides a technical overview of Agilent PLgel 5 µm MIXED-C Columns GPC calibrations and hydrodynamic volume. When calibrating the same column with standards of different chemical composition, the shape of the calibration should be similar. The plots will differ according to the hydrodynamic volume of each polymer in the element used.

The PLgel 5 µm MIXED-C columns are designed for rapid polymer analysis and are ideal for comparing batches of polycarbonate. With its linear calibration up to 2 million MW, PLgel 5 µm MIXED-C is the column of choice for highest resolution and accuracy in molecular weight distribution analyses.

This document introduces the technical overview of Agilent PLgel 5 µm MIXED-C columns, including the adsorption phenomena in organic GPC separations and how to minimize this impact by modifying the eluent polarity.

This document describes the use of Varian CP-4900 Micro GC and provides an example of how to use the EDU-Varian Sample Concentrator to concentrate samples to improve the detection limit.

This document introduces a method for analyzing polycarbonates using Agilent PLgel 5 µm MiniMIX-C columns that can reduce the amount of solvent used.

This document describes a method for analyzing BTEX gas samples using the Varian 490-GC Micro GC. The method uses the EDU Sample Concentrator to concentrate the sample and then injects the sample into the GC for separation and analysis. Using this method, enrichment factors of up to 230 times can be achieved.

This file introduces the advantages of Varian nano-Electrospray Ionization Source (nESI), which can be connected to all current Varian LC/MS systems. nESI can improve the ionization efficiency of the sample, make the droplets in the sample solution smaller, reduce the contribution of solvent to the background noise, and thus improve the sensitivity. nESI can also be combined with 75-micron diameter columns and nano-flow LC pumps to increase sample concentration and sensitivity. nESI is suitable for low sample volume applications such as proteomics research because it consumes less sample.

The document describes the process of seamlessly transferring validated methods from the Agilent 1200 Series LC system to the Agilent 1220 and 1260 Infinity LC systems. The method used USP/NF 23 method and the system suitability test developed on an Agilent 1200 Series LC system was used to test whether the Agilent 1220 and Agilent 1260 Infinity LC systems met the acceptance criteria. The chromatographic conditions of the system suitability test transferred to the Agilent 1260 and Agilent 1220 Infinity LC systems without changes. The results obtained were consistent with the results typically obtained on the Agilent 1200 Series LC system.

Introduces the characteristics and advantages of Agilent PL aquagel-OH 50 8 μm column for the separation of water-soluble polymers.

This document provides a technical overview of Agilent PL aquagel-OH 40 8 µm columns, which are suitable for determining the molecular weight distributions of water-soluble polymers and for separating oligomers and small molecules. These columns have an extremely hydrophilic and neutral surface, allowing for high-performance analysis of analytes with neutral, ionic, and hydrophobic moieties. The PL aquagel-OH 40 8 µm high-performance columns are ideal for medium molecular weight separations, offering high pore volume and high column efficiency.

This document describes the features and characteristics of Agilent PL aquagel-OH 40 8 μm high performance columns, which are ideal for medium molecular weight separations of polysaccharides. They combine high pore volume and high column efficiency for maximum resolution.

This document introduces the application of PL aquagel-OH 60 8 μm and PL aquagel-OH 40 8 μm high performance columns for relatively high molecular weight aqueous separations of methyl cellulose by size exclusion chromatography (SEC).

This document introduces the Agilent PL aquagel-OH MIXED-H 8 μm columns, which provide high resolution (>35,000 plates/m) over a very wide range of molecular weights, simplifying column selection and providing a flexible analytical system. Due to the extremely hydrophilic nature of these columns, neutral polymers such as polyethylene glycol/oxide (PEG/PEO) can be run with pure water as the eluent, using size exclusion chromatography (SEC). Figure 1 shows a typical raw data chromatogram for a mixture of Agilent PEO/PEG standards. The broad molecular weight resolving range of the PL aquagel-OH MIXED-H 8 μm column is clearly evident.

This document describes the features of Agilent PL aquagel-OH 30 8 μm high performance columns. The columns are highly hydrophilic and suitable for separations using water as the solvent. PL aquagel-OH columns are ideal for low molecular weight separations by size exclusion chromatography, offering high pore volume and high column efficiency.