This document introduces a method for analyzing polycarbonates using Agilent PLgel 5 µm MiniMIX-C columns that can reduce the amount of solvent used.
This document provides a technical overview of Agilent PLgel 5 um MiniMIX-C columns, which are designed to reduce hazardous solvent consumption during polycarbonate analysis. By using narrow bore columns, chloroform usage can be significantly lowered while maintaining high performance comparable to conventional ID columns.
The document specifies recommended operating conditions, including a flow rate of 0.3 mL/min and a sample loading of 0.1% in 20 uL. These columns offer benefits such as increased operator safety, reduced solvent disposal costs, and excellent mechanical stability when used with conventional GPC equipment.
| Column Dimensions | 250 x 4.6 mm |
|---|---|
| Particle Size | 5 um |
| Recommended Flow Rate | 0.3 mL/min |
| Recommended Eluent | Chloroform |
| Sample Loading | 0.1%, 20 uL |
| Detection Method | RI |
| Solvent Reduction | ~ 70% |
They achieve significantly lower solvent consumption, increased operator safety, and reduced solvent disposal costs.
To maintain the same linear velocity through the column, the volumetric flow rate must be reduced to 0.3 mL/min.
Two columns are recommended per set, with dimensions of 250 x 4.6 mm.
The eluent specified for the analysis is chloroform.