This file introduces the advantages of Varian nano-Electrospray Ionization Source (nESI), which can be connected to all current Varian LC/MS systems. nESI can improve the ionization efficiency of the sample, make the droplets in the sample solution smaller, reduce the contribution of solvent to the background noise, and thus improve the sensitivity. nESI can also be combined with 75-micron diameter columns and nano-flow LC pumps to increase sample concentration and sensitivity. nESI is suitable for low sample volume applications such as proteomics research because it consumes less sample.
The Varian nano-Electrospray Ionization Source (nESI) is an interface designed to add nano-flow capability to existing Varian LC/MS systems. It extends the flow rate range to less than 100 nL/min, offering enhanced sensitivity through higher ionization efficiency and smaller droplets while reducing sample consumption.
The nESI extends the flow rate range to less than 100 nL/min.
The nESI is designed to be used with 75-micron diameter columns.
The optimization process shows voltage settings of 1200 V, 1300 V, and 1400 V.