This document provides a technical overview of Agilent PLgel 5 µm MIXED-C Columns GPC calibrations and hydrodynamic volume. When calibrating the same column with standards of different chemical composition, the shape of the calibration should be similar. The plots will differ according to the hydrodynamic volume of each polymer in the element used.
This document provides a technical overview regarding Gel Permeation Chromatography (GPC) calibrations. It explains that when the same column is calibrated with standards of different chemical compositions, the resulting calibration plots will differ based on the hydrodynamic volume of each polymer element used.
The text illustrates this effect using polytetrahydrofurans and polystyrenes as standards on an Agilent PLgel 5 um MIXED-C column. A figure displays retention time versus calibrant molecular weight for these materials.
The column measures 380 x 7.5 mm.
The part number is PL1110-6500.