This document introduces the technical overview of Agilent PLgel 5 µm MIXED-C columns, including the adsorption phenomena in organic GPC separations and how to minimize this impact by modifying the eluent polarity.
This document provides a technical overview regarding the use of eluent modification with Agilent PLgel 5 um MIXED-C columns. It addresses adsorption phenomena observed in organic GPC separations, specifically for polymer/solvent systems like polylactic acid in chloroform.
The text explains that modifying the eluent (e.g., adding methanol to chloroform) increases solvent polarity and minimizes solute/column interaction. The document confirms that PLgel columns are fully compatible with this modification, including water as a modifier up to about 10% by volume.
The PLgel 5 um MIXED-C columns provide a linear calibration up to 2 million MW.
PLgel columns are fully compatible with water as a modifier up to about 10% by volume.
The recommended flow rate for this analysis is 1.0 mL/min.