The document describes the process of seamlessly transferring validated methods from the Agilent 1200 Series LC system to the Agilent 1220 and 1260 Infinity LC systems. The method used USP/NF 23 method and the system suitability test developed on an Agilent 1200 Series LC system was used to test whether the Agilent 1220 and Agilent 1260 Infinity LC systems met the acceptance criteria. The chromatographic conditions of the system suitability test transferred to the Agilent 1260 and Agilent 1220 Infinity LC systems without changes. The results obtained were consistent with the results typically obtained on the Agilent 1200 Series LC system.
This document provides a technical overview of transferring validated chromatographic methods from the Agilent 1200 Series LC system to the newer Agilent 1220 Infinity and Agilent 1260 Infinity LC systems. It demonstrates that results obtained on the new instruments comply with acceptance criteria established for the original system without requiring method revalidation.
The retention time shift for all peaks was less than 5% when using the same column and mobile phase batch.
The precision of areas must be less than 2% relative standard deviation.
The precision of retention times must be less than 0.5% relative standard deviation.