This document introduces the Agilent PL aquagel-OH MIXED-H 8 μm columns, which provide high resolution (>35,000 plates/m) over a very wide range of molecular weights, simplifying column selection and providing a flexible analytical system. Due to the extremely hydrophilic nature of these columns, neutral polymers such as polyethylene glycol/oxide (PEG/PEO) can be run with pure water as the eluent, using size exclusion chromatography (SEC). Figure 1 shows a typical raw data chromatogram for a mixture of Agilent PEO/PEG standards. The broad molecular weight resolving range of the PL aquagel-OH MIXED-H 8 μm column is clearly evident.
The document provides a technical overview of the Agilent PL aquagel-OH MIXED-H 8 um columns, highlighting their high resolution and ability to simplify column selection. These hydrophilic columns allow for the analysis of neutral polymers like PEG/PEQ using pure water as an eluent in size exclusion chromatography.
| Resolution | >35,000 plates/m |
|---|---|
| Particle Size | 8 um |
| Column Dimensions | 300 x 7.5 mm |
| Part Number | PL1149-6800 |
| Eluent | Water |
| Flow Rate | 1 mL/min |
| Detection Method | RI |
The columns provide high resolution of greater than 35,000 plates per meter.
Pure water can be used as the eluent for these neutral polymers.
The column is 300 x 7.5 mm and has the part number PL1149-6800.