Agilent Manuals

This application note demonstrates the capabilities of the Agilent J&W CP-COX column with the Agilent 490 Micro GC, including separation of permanent gases and backflush possibilities to ensure extended column lifetimes.

Two samples consisting of various nonsteroidal anti-inflammatory drugs (NSAID) are separated using methods developed on an Agilent 1200 Series Method Development Solution controlled by ACD/Labs AutoChrom for ChemStation software. The chromatograph while under control of AutoChrom can screen up to seven columns, thirteen buffers and two organic solvents with columns held in four temperature controlled zones. The software helps plan the next best experiment to perform, allowing the analyst to focus on quickly developing methods using conditions with the best likelihood of success. In this work four columns (Agilent ZORBAX StableBond SB-C18, Agilent ZORBAX Eclipse Plus C18, Agilent ZORBAX Bonus RP and Agilent ZORBAX Eclipse Plus Phenyl-Hexyl) are screened using five mobile phase modifiers. Twenty solvent column experiments are initially screened for each sample set.

This application note introduces novel statistical methods for determining the absolute structure of light-atom organic molecules. All of these methods require precise experimental determination of very small differences in the intensities of related reflections, which is facilitated by Agilent’s micro-focus SuperNova X-ray diffractometer.

This document presents the application of GPC/SEC for the analysis of polyolefins. It covers the GPC separation mechanism, system requirements for polyolefin analysis, sample preparation, system, software and standards, as well as GPC analysis of different types of polyolefins.

This article introduces a method for studying the negative thermal expansion of metal cyanides using variable temperature powder diffraction.

This article describes the application of CrysAlisPro software tools in handling data sets of non-Merohedral crystals. By using the software tools of CrysAlisPro, it is relatively easy to obtain high-quality structures.

This Application Note introduces the “Follow sudden changes of sample orientation” tool in CrysAlisPro’s data reduction software. This tool can track crystal movement and correct for the data affected by it.

This document describes the use of Xcalibur PX Ultra to collect highly redundant data from cubic insulin at York Structural Biology Laboratory, demonstrating protein structure phasing using the sulfur anomalous signal. The document provides details on the data collection, indexing, integration, and refinement processes.

Gemini A is a dual wavelength diffractometer suitable for modern small molecule crystallography laboratories. Designed and brought to you by the world’s leading manufacturers of dual wavelength systems, the Gemini A is a compact and easy-to-use instrument combining the versatility and benefits of co-mounted both molybdenum and copper Enhance X-ray sources on a single 4-circle kappa goniometer.

This paper introduces a new type of X-ray source for the characterization of the crystal structures of derivatized resorcarenes, which has both Mo and Cu X-ray radiation sources, and experimentally verifies its effectiveness in the characterization of the crystal structures of derivatized resorcarenes.

This document describes the results of trace-level PAH analyses using Agilent J&W DB-5ms Ultra Inert 30 m × 0.25 mm, 0.25 µm capillary GC column on a 16-component PAH standard mixture.

This application demonstrates the use of electron impact GC–MS for trace-level PBDE analysis. The method uses Agilent J&W DB-5ms Ultra Inert GC column. This column has been thoroughly tested for column inertness and provides the analyst with high confidence in the accuracy of the results.

Highly Sensitive UV Analysis with the Agilent 1290 Infinity LC System for Fast and Reliable Cleaning Validation – Part 3 Determination of residual active pharmaceutical impurities from a previous production batch using a DAD with standard or high sensitivity flow cell

The PX Scanner is a revolutionary product from Agilent Technologies designed specifically for the in-situ identification and analysis of protein crystals within multi-well crystallisation plates. This application note presents a collection of experimental results obtained using the PX Scanner.

The Xcalibur E is a fully automated X-ray crystallography instrument for crystal to structure applications. It features a user-friendly interface and allows for fast and accurate chemical structure determination. Unlike other bench-top instruments that are limited to studying routine large crystals, the Xcalibur E is capable of handling crystals of various sizes and qualities. With its compact design and upgrade options for dual wavelength and CCD, it provides high-quality crystal structures with minimal user intervention in a short amount of time.