Agilent Manuals

Fast (5 min), dilute-and-shoot method for the determination of morphine and one of its major metabolites (morphine-glucuronide) in biological fluids (saliva and urine) using the Agilent 500 Ion Trap LC/MS

This application note presents a method for separating peptides using acetonitrile-free gradient elution on SepTech ST150 10-C18. In response to the global shortage of acetonitrile, alternative solvents such as ethanol have been investigated, and the performance of SepTech ST150 10-C18 is compared to three leading competitor materials. The results demonstrate the superior resolution of peptides achieved by SepTech ST150 10-C18 under acetonitrile-free mobile phase conditions.

This application note details a quick and effective analytical method for the determi- nation of low ppm and trace level organophosphorus (OP) pesticide residues in an olive oil extract.

The application note demonstrates how the Cary 60 is used to measure the purity of DNA at 4 °C using the micro fiber optics accessory – resulting in significant time and cost savings without compromise in the accuracy and reproducibility of data quality.

This document highlights the method and advantages of using the Agilent Cary 60 UV-Vis spectrophotometer for determining drug tablet concentration in pharmaceutical applications.

This article investigates the photo-kinetics of a platinum organoamine complex using the Cary 50/60 Application Note. A photochemical reaction is initiated by the absorption of light, leading to subsequent reactions and product formation.

This document describes the use of the quartz fiber optic dip probe on the Cary 50/60 UV-Vis spectrophotometer to analyze the nitrate content in water.

This document describes the analysis of epoxy resins using Agilent PLgel MIXED-E with Gel Permeation Chromatography. Epoxy resins are thermosetting copolymers that are widely used as general-purpose adhesives, coatings, and composites due to their durability. The analysis is straightforward with Agilent PLgel 3 µm MIXED-E columns, which can analyze low molecular weight samples as well as polymers up to 30,000 MW.

This is a product usage manual for analyzing polyester polyols with gel permeation chromatography using the Agilent PLgel MIXED-E column.

This document introduces the application of Agilent PLgel MIXED-E gel permeation chromatography technology in the analysis of phenolic resins. Gel permeation chromatography of phenolic resins can be easily performed using Agilent PLgel 3µm MIXED-E columns, which are suitable for both low molecular weight samples and high molecular weight samples. In addition, for more difficult polar resins, Agilent PolarGel columns are preferred for analysis.

This document describes the structure of a Titin/Obscurin-like 1 complex solved using an Agilent Xcalibur Nova X-ray Diffractometer, providing structural insights into muscle M-band assembly and mechanics.

A new reverse phase media, Agilent ZORBAX RRHD 300SB-C18 1.8µm, was used for the separation of a typical protein biopharmaceutical, insulin.

This Application Note demonstrates the influence of system delay volume and system dispersion on the resulting resolution and sensitivity achieved for LC/MS experi- ments. The advantages of using a low delay volume LC system and optimized connec- tion of the LC to the MS are shown. The study illustrates that the Agilent 1290 Infinity LC system is an ideal partner for LC/MS instruments.

This Application Note shows the analysis of resveratrol in red wine using the Agilent 1290 Infi nity LC. The fast and highly sensitive analysis of resveratrol was done on an Agilent 1290 Infi nity LC using an Agilent ZORBAX RRHD sub-2 µm column and the Max-Light high-sensitivity fl ow cell with 60 mm path length.

This application note describes the use of an Agilent HPLC-Chip system coupled to an Agilent Accurate-Mass Q-TOF LC/MS system for analysis of in-process samples of recombinant protein production. Combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, the superior mass accuracy of the HPLC-Chip LC/MS platform enabled identifi cation of impurities during the production process. This type of analysis aids in the assessment of impurities found in protein pharmaceutical at the early phases of product development.