Agilent Manuals

This Application Note presents a workflow for the identification of main drug metabolites by sample comparison as well as by structure elucidation of the identified metabolites based on accurate MS and MS/MS data acquired with a quadrupole time-of-flight (Q-TOF) LC/MS. In pharmaceutical drug development the main metabolites of a new drug candidate must be identified as fast as possible and with high confidence. Software for this task is described, which analyzes the metabolized drug samples and visualizes the differences with non- metabolized drug samples for manual investigation. The structures of possible metabolites are elucidated by examination of the original highly-accurate Q-TOF mass data based on the differences between the metabolized and non-metabolized drug samples.

Microfluidic chips for detecting microsatellite instability

Optimizing the Agilent 1100 Series well-plate sampler

Using two identical columns and a second pump can achieve high-throughput analytical HPLC.

The Agilent 1200 Series Rapid Resolution LC system can operate continuously to meet the stringent requirements of multiple medicinal chemists for a high-throughput LC/MS system.

This application note describes the use of the Agilent 1100 Series LC system for the analysis of β-blockers in blood and other biological fluids using online and offline sample preparation methods.

Using an Agilent 1200 Series LC system, featuring the G1316B Thermostatted Column Compartment, is used to generate reproducible chromatography with a programmable tem- perature gradient

The document compares the performance of Agilent Technologies' new series of instruments, the 7890 Series GC and 5975 Series MSD, to the previous versions, the 6890 Series GC and the 5973 Series MSD. Performance is shown to be fully equivalent in test comparisons using a 16-component polynuclear aromatic hydrocarbon (PAH) sample. The document also discusses the reliable transfer of existing methods from the 6890/5973 system to the new 7890/5975 system.

This Application Note describes the use of a high-throughput rapid resolution LC/MS TOF system for detection of impurities in a pharmaceutical formulation in more than 1000 samples per day. The instrument setup and a method for alternating column regeneration in a high-throughput LC system to reduce the overall analysis time by 50 % are also described.

This document introduces the use of the Agilent 1200 Series liquid chromatography (LC) system coupled to the 6410 Triple Quadrupole Mass Spectrometer (QQQ) by way of the G1948B electrospray ionization (ESI) source, for the analysis of anabolic substances in urine.

This document introduces Agilent's GC and LC column selection guides, which make it easier for customers to select the right columns using simple guides. Comprehensive column choices improve lab productivity, reduce method development time, and improve results.

This document is a compendium of HPLC analysis methods for traditional Chinese medicine and chemical drugs in the China Pharmacopoeia. It includes HPLC analysis methods for traditional Chinese medicine from Volume I and chemical drugs from Volume II of the 2005 edition of the China Pharmacopoeia. The document highlights the selectivity and sensitivity of HPLC as an analysis technique, as well as its widespread adoption in the China Pharmacopoeia.

This Application Note describes the analysis of different antibody sam- ples under reducing and non-reducing conditions.

This application note describes a method for analyzing antibody samples using an Agilent 1200 series HPLC coupled with a 6210 TOF MS. The results indicate that this method can quickly and accurately determine the molecular weight (MW) of antibodies and can resolve the various antibody subpopulations present in a sample.

This document discusses the risks of residual solvents in pharmaceutical products and the regulatory requirements from national and international bodies. The use of Agilent 7890A GC and Agilent G1888 automated headspace sampler allows for sensitive and accurate analysis of solvent residues. The document provides details on experimental conditions and instrument parameters.