Agilent Manuals

This application note describes a method for the analysis of volatile organic compounds (VOCs) in water using static headspace-GC/MS, including method optimization, sample preparation, and analytical method.

This application note describes how to use the Agilent 7000A Triple Quadrupole GC/MS System to determine nitro-polycyclic aromatic hydrocarbons (nitro-PAHs) in air particulates

This Application Note describes a rapid method for analysis of polynuclear aromatic hydrocarbons (PAHs) in soil and sediment samples by GC-MS using an Agilent Low Thermal Mass (LTM) GC/MS system and Capillary Flow Technology QuickSwap for Backflush. The cycle time for GC-MS analysis of PAHs was dramatically improved through the combined use of a narrow-bore (180-µm id) column installed in an LTM module and capillary column back-flushing with QuickSwap. Improvements were achieved while maintaining resolution. Agilent Capillary Flow Technology enabled column backflushing to remove low-volatili- ty material from the capillary column, reducing bakeout and maintenance time. The method resulted in increased sample throughput and lab productivity, while maintain- ing analytical performance.

Agilent has further improved the sample throughput capabilities of its 7500cx ICP-MS with Octopole Reaction System (ORS) using a newly configured Integrated Sample Introduction System–Discrete Sampling (ISIS-DS) accessory, and helium collision mode. Employing this new methodology, a complete suite of 30 or more elements can be analyzed in compliance with USEPA criteria (spectrum mode, three replicates, and sub-ppb MDLs) in approximately 75 seconds, sample to sample, with excellent removal of polyatomic interferences. Performance data showing stability, interference control, accuracy, precision, and washout are presented.

This collection of Application Briefs demonstrates the wide range of analyses that can be successfully addressed with the Agilent TD-GC/MS systems, including monitoring air toxics, monitoring hazardous air pollutants, soil gas and vapor-intrusion assessments, sorbent tube stack emissions testing, occupational hygiene/workplace air monitoring, fugitive emissions, odor, and fence-line monitoring, and indoor air quality.

This application note demonstrates the performance of the Agilent 1290 Infinity LC System coupled to an Agilent 6460A Triple Quadrupole LC/MS for the evaluation of metabolic stability using the cassette analysis approach combined with fast polarity switching.

Quantitative proteomics using multiple reaction monitoring (MRM) has emerged as an important methodology for biomarker validation. Multiple-reaction monitoring on a triple quadruple (QQQ) mass spectrometer provides superior sensitivity and selec- tivity for targeted peptides in a complex sample. MRM also offers high precision in quantitation and a fast scan speed, which makes it an ideal technology for validating biomarkers in a high-throughput fashion. This application note demonstrates the entire workflow for biomarker validation offered by Agilent Technologies using multiple reaction monitoring together with statistical analysis software. Thousands of peptide transitions were generated using Agilent Q-TOF and Spectrum Mill MRM selector. The transition list was imported into MassHunter acquisition software equipped with dynamic MRM functionality and monitored in a single LC/MS run. Finally, MRM quantitation results were analyzed using Mass Profiler Pro for principle component analysis (PCA), hierarchical clustering and ANOVA analysis.

This article describes the quantification of potential genotoxic impurity D in Atenolol using LC/ESI/MS/MS method with Agilent 1200 Series RRLC and 6410B Triple Quadrupole LC/MS instruments. The developed method is simple, shorter in runtime, with better resolution and higher sensitivity than the existing European and United States Pharmacopeia methods. Agilent's MassHunter Optimization software was used for quick optimization of MS parameters. By connecting the Agilent 6410B Triple Quadrupole (QQQ) LC/MS detector to an Agilent 1200 Series RRLC system, the quantification range can be effectively reduced further down to 5 ppb with a linearity coefficient greater than 0.9992.

This system addresses the challenges faced by laboratories performing toxicology screens on forensic samples by combining fast GC to reduce run time, simultaneous collection of scan, SIM, and NPD data in one shorter run, backflushing to prevent detector fouling, and Deconvolution Reporting Software (DRS) to simplify data interpretation. The system is able to find all the molecules detected by the conventional method in significantly less time.

This note introduces the methods for chiral separations by CE, including theory, method development strategies, experimental procedures etc.

This paper introduces a method for optimizing the separation of organic acids in fruit juice using low-pH ZORBAX Stable-Bond SB-Aq columns. The use of short columns, little organic solvent, and low-pH mobile phase enables rapid separation of organic acids in fruit juice.

Lipidomics discovery profiling and targeted LC/MS analysis in 3T3-L1 differentiating adipocytes

This document introduces the application of capillary electrophoresis in peptide analysis, including protein identification, peptide mapping, and purity checking of synthetic peptides. The authors describe the methodological aspects of peptide analysis, including the optimization of protein digestion buffers, electrophoretic conditions, reproducibility, detection sensitivity, and spectral analysis.

This article describes the capillary zone electrophoresis analysis of neuropeptides. The method allows for rapid, sensitive, and simultaneous identification and quantification of diastereomeric peptides.

This document describes how to use Agilent ZORBAX Eclipse Plus C18 5µm, 3.5µm and 1.8µm columns for simvastatin analysis